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Molecular characterization of avian pathogenic Escherichia coli strains in Jiangxi Province, China, and development of polyvalent inactivated vaccines

文献类型： 外文期刊

作者： Tan, Jia 1 ; Wang, Ying-Xin 3 ; Kang, Zhao-Feng 1 ; Huang, Jiang-Nan 1 ; Li, Hai-Qin 1 ; Wu, Cheng-Cheng 1 ; Li, Na 1 ; Zhang, Fan-Fan 1 ; Tan, Mei-Fang 1 ;

作者机构： 1.Jiangxi Acad Agr Sci, Inst Anim Husb & Vet Sci, Nanchang 330200, Peoples R China

2.Minist Agr & Rural Affairs, Jiangxi Prov Key Lab Green & Hlth Breeding Livesto, Nanchang 330200, Peoples R China

3.Anim Dis Prevent & Control Ctr, Ezhou 436000, Peoples R China

关键词： Avian pathogenic Escherichia coli; Whole-genome sequencing; Virulence-associated gene; Serotype; Vaccine

期刊名称：POULTRY SCIENCE （ 影响因子：4.2； 五年影响因子：4.5 ）

ISSN： 0032-5791

年卷期： 2025 年 104 卷 2 期

页码：

收录情况： SCI

摘要： Avian pathogen Escherichia coli (APEC) poses a significant threat to poultry farming, causing colibacillosis in various hosts, including chickens, ducks, geese, and pigeons. This study aimed to investigate the molecular epidemiology of APEC in Jiangxi Province, China, to inform prevention and control strategies. Between 2020 and 2024, 186 APEC strains were isolated and identified, with 88.7 % from liver samples and 5.4 % from brain tissues. Whole-genome sequencing and analysis revealed that serotypes O145, O78, O8, and O111 comprised 32.9 %, 9.3 %, 7.9 %, and 4.3 % of the isolates, respectively. The dominant H serotypes included H21 (23.8 %), H9 (22.7 %), and H4 (18.2 %), with ST23 being the most prevalent sequence type at 24.2 %, followed by ST117 (10.1 %) and ST2505 (7.3 %). Virulence-associated genes in the strains O145, O78, O8, and O111 were further analyzed for quantity and pattern. Eight strains were selected for in vivo pathogenicity testing in ducks, four highly pathogenic strains were then identified, which were used to develop inactivated vaccines with the novel aqueous adjuvant MONTANIDETM GEL P PR and the traditional adjuvant alumVax hydroxide. Compared with existing vaccines, those formulated in this study showed superior protection, with the MONTANIDETM GEL P PR adjuvant achieving the highest protection rate (90.0 %, 36/40) and lower lesion scores. This research enhances the understanding of APEC epidemiology and offers promising strategies for effective vaccine development against prevalent APEC.

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