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Analysis of the Difenoconazole-Resistance Risk and Its Molecular Basis in Colletotrichum truncatum from Soybean

文献类型： 外文期刊

作者： Shi, Niuniu 1 ; Qiu, Dezhu 1 ; Chen, Furu 1 ; Yang, Ying-Qing 3 ; Du, Yixin 1 ;

作者机构： 1.Fujian Acad Agr Sci, Inst Plant Protect, Fuzhou 350013, Fujian, Peoples R China

2.Fujian Key Lab Monitoring & Integrated Management, Fuzhou 350013, Fujian, Peoples R China

3.Jiangxi Acad Agr Sci, Inst Plant Protect, Nanchang, Jiangxi, Peoples R China

关键词： Colletotrichum truncatum; CYP51; difenoconazole; I463V; resistance mechanism; soybean

期刊名称：PLANT DISEASE （ 影响因子：4.5； 五年影响因子：5.0 ）

ISSN： 0191-2917

年卷期： 2023 年

页码：

收录情况： SCI

摘要： Anthracnose disease, caused by Colletotrichum truncatum, is a destructive fungal disease in soybean worldwide, and some demethylation inhibitor fungicides are used to manage it. In this study, the sensitivity of C. truncatum to difenoconazole was determined, and the risk for resistance development of C. truncatum to difenoconazole was also assessed. The results showed that the mean EC50 value was 0.9313 mu g/ml, and the frequency of sensitivity formed a unimodal distribution. Six stable mutants with a mutation frequency of 8.33 x 10(-5) were generated, and resistance factors ranged from 3.00 to 5.81 after 10 successive culture transfers. All mutants exhibited fitness penalties in reduced mycelial growth rate, sporulation, and pathogenicity, except for the Ct2-3-5 mutant. Positive cross-resistance was observed between difenoconazole and propiconazole but not between difenoconazole and prochloraz, pyraclostrobin, or fluazinam. One point mutation I463V in CYP51A was found in five resistant mutants. Surprisingly, the homologous I463V mutation has not been observed in other plant pathogens. CYP51A and CYP51B expression increased slightly in the resistant mutants as compared to wild-types when exposed to difenoconazole but not in the CtR61-2-3f and CtR61-2-4a mutants. In general, a new point mutation, I463V in CYP51A, could be associated with low resistance to difenoconazole in C. truncatum. In the greenhouse assay, control efficacy of difenoconazole on both parental isolates and the mutants increased in a dose-dependent manner. Collectively, the resistance risk of C. truncatum to difenoconazole is regarded to be low to moderate, suggesting that difenoconazole can still be reasonably used to control soybean anthracnose.

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