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The Eukaryote-Like Serine/Threonine Kinase STK Regulates the Growth and Metabolism of Zoonotic Streptococcus suis

文献类型： 外文期刊

作者： Zhang, Chunyan 1 ; Sun, Wen 1 ; Tan, Meifang 2 ; Dong, Mengmeng 1 ; Liu, Wanquan 1 ; Gao, Ting 3 ; Li, Lu 1 ; Xu, Zhuofei 1 ;

作者机构： 1.Huazhong Agr Univ, Coll Vet Med, State Key Lab Agr Microbiol, Wuhan, Peoples R China

2.Jiangxi Acad Agr Sci, Inst Anim Husb & Vet Med, Vet Med Lab, Nanchang, Jiangxi, Peoples R China

3.Hubei Acad Agr Sci, Inst Anim Husb & Vet Sci, Vet Med Lab, Wuhan, Peoples R China

4.Cooperat Innovat Ctr Sustainable Pig Prod, Wuhan, Peoples R China

关键词： Streptococcus suis;eukaryote-like;serine/threonine kinase;phosphorylation;RNA-Seq;phosphoproteome;growth;virulence;metabolism

期刊名称：FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY （ 影响因子：5.293； 五年影响因子：5.882 ）

ISSN： 2235-2988

年卷期： 2017 年 7 卷

页码：

收录情况： SCI

摘要： Like eukaryotes, bacteria express one or more serine/threonine kinases (STKs) that initiate diverse signaling networks. The STK from Streptococcus suis is encoded by a single-copy stk gene, which is crucial in stress response and virulence. To further understand the regulatory mechanism of STK in S. suis, a stk deletion strain (1 stk) and its complementary strain (C Delta stk) were constructed to systematically decode STK characteristics by applying whole transcriptome RNA sequencing (RNA-Seq) and phosphoproteomic analysis. Numerous genes were differentially expressed in Delta stk compared with the wild-type parental strain SC-19, including 320 up-regulated and 219 down-regulated genes. Particularly, 32 virulence-associated genes (VAGs) were significantly down-regulated in Delta stk. Seven metabolic pathways relevant to bacterial centralmetabolismand translation are significantly repressed in Delta stk. Phosphoproteomic analysis further identified 12 phosphoproteins that exhibit differential phosphorylation in Delta stk. These proteins are associated with cell growth and division, glycolysis, and translation. Consistently, phenotypic assays confirmed that the Delta stk strain displayed deficient growth and attenuated pathogenicity. Thus, STK is a central regulator that plays an important role in cell growth and division, as well as S. suis metabolism.

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