文献类型： 外文期刊

作者： Ding, Xia ¹ ; Peng, Xiao-Jue ¹ ; Li, Wen-Zhuo ¹ ; Cai, Yao-Hui ² ; Yang, Wei-Jun ³ ;

作者机构： 1.Nanchang Univ, Coll Life Sci, Nanchang 330031, Jiangxi, Peoples R China

2.Jiangxi Acad Agr Sci, Nanchang 330000, Jiangxi, Peoples R China

3.Zhejiang Univ, Coll Life Sci, Hangzhou 310058, Zhejiang, Peoples R China

关键词： Methanothermobacter;polymerase chain reaction (PCR) melting profile;protein fingerprint profile analyses

期刊名称：AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH （ 影响因子：0.539； 五年影响因子：0.564 ）

ISSN： 1996-0808

年卷期： 2011 年 5 卷 26 期

页码：

收录情况： SCI

摘要： A thermophilic new strain of Methanothermobacter marburgensis DX01, with a different morphology from M. thermautotrophicus delta H, was isolated from a hot spring in China. To provide further molecular evidence to identify the methanogen M. marburgensis DX01, polymerase chain reaction melting profile (PCR MP) technique and protein fingerprint profiling were performed. Both DNA and protein fingerprint profiles showed significant differences between M. marburgensis DX01 and M. thermautotrophicus delta H. In addition, five interesting DNA fragments obtained from PCR MP of the DX01 strain were excised and sequenced. We found that all five DNA fragments were unique to M. marburgensis DX01. Expression of induced proteins in M. marburgensis DX01 was analyzed by protein fingerprint profiling and a protein that was more highly expressed at 70 degrees C was excised and identified by N-terminal amino acid sequencing. We found that the up-regulated protein was Methyl-coenzyme M reductase., which was 97.6% amino acid sequence identity with that of M. thermautotrophicus delta H. These studies may provide clues for a better understanding of the new isolate of thermophilic M. marburgensis.