文献类型： 外文期刊
作者： Qiu, Suyan 1 ; Lin, Zhenyu 3 ; Zhou, Yaomin 1 ; Wang, Donggen 1 ; Yuan, Lijuan 1 ; Wei, Yihua 1 ; Dai, Tingcan 1 ; Luo, L 1 ;
作者机构： 1.Jiangxi Acad Agr Sci, Inst Qual & Safety & Stand Agr Prod Res, Nanchang 330200, Jiangxi, Peoples R China
2.Fuzhou Univ, Dept Chem, Fujian Prov Key Lab Anal & Detect Technol Food Sa, MOE Key Lab Anal & Detect Food Safety, Fuzhou 350002, Fujian, Peoples R China
3.Fuzhou Univ, Dept Chem, Fujian Prov Key Lab Anal & Detect Technol Food Sa, MOE Key Lab Anal & Detect Food Safety, Fuzhou 350002,
期刊名称：ANALYST （ 影响因子：4.616； 五年影响因子：4.232 ）
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收录情况： SCI
摘要： A rapid and cost-effective colorimetric sensor has been developed for the detection of bacteria (Bacillus subtilis was selected as an example). The sensor was designed to rely on lysozyme-capped AuNPs with the advantages of effective amplification and high specificity. In the sensing system, lysozyme was able to bind strongly to Bacillus subtilis, which effectively induced a color change of the solution from light purple to purplish red. The lowest concentration of Bacillus subtilis detectable by the naked eye was 4.5 x 10(3) colony-forming units (CFU) mL(-1). Similar results were discernable from UV-Vis absorption measurements. A good specificity was observed through a statistical analysis method using the SPSS software (version 17.0). This simple colorimetric sensor may therefore be a rapid and specific method for a bacterial detection assay in complex samples.
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