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Label-Free Fluorometric Method for Monitoring Conformational Flexibility of Laccase Based on a Selective Laccase Sensor

文献类型： 外文期刊

作者： Qiu, Suyan 1 ; Lin, Zhenyu 2 ; Zhou, Yaomin 1 ; Li, Ruili 1 ; Zhang, Jinyan 1 ; Zhang, Dawen 1 ; Luo, Linguang 1 ; Guo, L 1 ;

作者机构： 1.Jiangxi Acad Agr Sci, Inst Qual & Safety & Stand Agr Prod Res, Nanchang 330200, Jiangxi, Peoples R China

2.Fuzhou Univ, Dept Chem, Fujian Prov Key Lab Anal & Detect Technol Food Sa, MOE Key Lab Anal & Detect Food Safety, Fuzhou 350002, Fujian, Peoples R China

3.Fuzhou Univ, Dept Chem, Fujian Prov Key Lab Anal & Detect Technol Food Sa, MOE K

期刊名称：ANALYTICAL CHEMISTRY （ 影响因子：6.986； 五年影响因子：6.755 ）

ISSN： 0003-2700

年卷期： 2013 年 85 卷 22 期

页码：

收录情况： SCI

摘要： A facile and selective fluorescence sensor for laccase determination has been proposed depending on the interaction between 3-azidocoumarin and trametes versicolor (Tv) laccase in this paper. The azido group of 3-azidocoumarin that is electron-rich alpha-nitrogen can directly interact with histidines that coordinate to three copper sites through hydrogen bonds and forms a new complex, which decreases the electron-donating ability of the azido group, leading to enhance the fluorescence intensity of the sensing system. Also, other common proteins have no significant interference for the proposed laccase sensor. Additionally, the proposed fluorescence sensor is extended to demonstrate the conformational flexibility of Tv laccase by the urea denaturant. A good consistency of the results obtained with the presented laccase sensor and CD spectra is performed. Furthermore, the relationship between the catalytic activity and the unfolding percentage of the unfolded Tv laccase through the proposed laccase sensor is also elucidated well.

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