Label-Free Fluorometric Method for Monitoring Conformational Flexibility of Laccase Based on a Selective Laccase Sensor
文献类型： 外文期刊
作者： Qiu, Suyan 1 ; Lin, Zhenyu 2 ; Zhou, Yaomin 1 ; Li, Ruili 1 ; Zhang, Jinyan 1 ; Zhang, Dawen 1 ; Luo, Linguang 1 ; Guo, L 1 ;
作者机构： 1.Jiangxi Acad Agr Sci, Inst Qual & Safety & Stand Agr Prod Res, Nanchang 330200, Jiangxi, Peoples R China
2.Fuzhou Univ, Dept Chem, Fujian Prov Key Lab Anal & Detect Technol Food Sa, MOE Key Lab Anal & Detect Food Safety, Fuzhou 350002, Fujian, Peoples R China
3.Fuzhou Univ, Dept Chem, Fujian Prov Key Lab Anal & Detect Technol Food Sa, MOE K
期刊名称：ANALYTICAL CHEMISTRY （ 影响因子：6.986； 五年影响因子：6.755 ）
ISSN： 0003-2700
年卷期： 2013 年 85 卷 22 期
页码：
收录情况： SCI
摘要： A facile and selective fluorescence sensor for laccase determination has been proposed depending on the interaction between 3-azidocoumarin and trametes versicolor (Tv) laccase in this paper. The azido group of 3-azidocoumarin that is electron-rich alpha-nitrogen can directly interact with histidines that coordinate to three copper sites through hydrogen bonds and forms a new complex, which decreases the electron-donating ability of the azido group, leading to enhance the fluorescence intensity of the sensing system. Also, other common proteins have no significant interference for the proposed laccase sensor. Additionally, the proposed fluorescence sensor is extended to demonstrate the conformational flexibility of Tv laccase by the urea denaturant. A good consistency of the results obtained with the presented laccase sensor and CD spectra is performed. Furthermore, the relationship between the catalytic activity and the unfolding percentage of the unfolded Tv laccase through the proposed laccase sensor is also elucidated well.
- 相关文献
作者其他论文 更多>>
-
CdTe based water-soluble fluorescent probe for rapid detection of zilpaterol in swine urine and pork
作者：Wei, Yihua;Li, Weihong;Han, Yan;Xiong, Yan;Kuang, Yuanying;Zhang, Jinyan
关键词：Zilpaterol hydrochloride; CdTe quantum dot; Fluorescence immunoassay
-
Response of the microbial community structure to the environmental factors during the extreme flood season in Poyang Lake, the largest freshwater lake in China
作者：Zhang, Li;Yuan, Lijuan;Xiang, Jianjun;Liao, Qiegen;Zhang, Dawen;Liu, Jutao
关键词：Poyang Lake; bacterial diversity; extreme flood season; environmental factors; microbial community
-
Spatio-temporal variation of toxin-producing gene abundance in Microcystis aeruginosa from Poyang Lake
作者：Wu, Lin;Zhang, Li;Yuan, Lijuan;Liao, Qiegen;Xiang, Jianjun;Zhang, Dawen;Wu, Lin;Qiu, Tong;Guo, Junhui;Liu, Jutao
关键词：Microcystis aeruginosa; mcyA; mcyB; qPCR; Relative expression
-
Accumulation characteristics and evaluation of heavy metals in soils and vegetables of plastic-covered sheds in typical red soil areas of China
作者：Nie, Genxin;Tu, Tianhua;Hu, Lifang;Zhou, Yaomin;Nie, Genxin;Tu, Tianhua;Hu, Lifang;Zhou, Yaomin;Wu, Ling
关键词：plastic-covered shed; red soil; vegetable; heavy metal accumulation; evaluation
-
Sensitive detection of cadmium ions based on a quantum-dot-mediated fluorescent visualization sensor
作者：Ai, Qiushuang;Dong, Yifan;Yu, Xiren;Zhang, Dawen;Qiu, Suyan;Wei, Peiling
关键词：
-
High-Performance Fluorescent Microspheres Based on Fluorescence Resonance Energy Transfer Mode for Lateral Flow Immunoassays
作者：Wang, Yumeng;Zhang, Gan;Xiao, Xiaoyue;Shu, Xinhui;Lai, Weihua;Fei, Dan;Guang, Yelan;Zhou, Yaomin
关键词：
-
Pharmacokinetics, withdrawal time, and dietary risk assessment of enrofloxacin and its metabolite ciprofloxacin, and sulfachloropyridazine-trimethoprim in Taihe black-boned silky fowls
作者：Yuan, Lijuan;Zhang, Li;Xiang, Jianjun;Liao, Qiegen;Luo, Linguang;Zhang, Dawen;Wu, Huizhen;Wang, Jianmei;Zhou, Min;Qian, Mingrong
关键词：antibiotics; dietary risk assessment; pharmacokinetics; Taihe black-boned silky fowl; withdrawal time
